Upregulation of miRNA-4776 in Influenza Virus Infected Bronchial Epithelial Cells Is Associated with Downregulation of NFKBIB and Increased Viral SurvivalReportar como inadecuado


Upregulation of miRNA-4776 in Influenza Virus Infected Bronchial Epithelial Cells Is Associated with Downregulation of NFKBIB and Increased Viral Survival


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1

Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA

2

School of Medicine, West Virginia University, Morgantown, WV 26506, USA





*

Author to whom correspondence should be addressed.



Academic Editor: Marc Kvansakul

Abstract Influenza A virus IAV infection remains a significant cause of morbidity and mortality worldwide. One key transcription factor that is activated upon IAV infection is nuclear factor Kappa B NF-κB. NF-κB regulation involves the inhibitor proteins NF-κB inhibitor beta NFKBIB, also known as IκB β, which form complexes with NF-κB to sequester it in the cytoplasm. In this study, microarray data showed differential expression of several microRNAs miRNAs on exposure to IAV. Target scan analysis revealed that miR-4776, miR-4514 and miR-4742 potentially target NFKBIB messenger RNA mRNA. Time-course analysis of primary bronchial epithelial cells HBEpCs showed that miR-4776 expression is increased within 1 h of infection, followed by its downregulation 4 h post-exposure to IAV. NFKBIB upregulation of miR-4776 correlated with a decrease in NFKBIB expression within 1 h of infection and a subsequent increase in NFKBIB expression 4 h post-infection. In addition, miRNA ago-immunoprecipitation studies and the three prime untranslated region 3’ UTR luciferase assay confirmed that miR-4776 targets NFKBIB mRNA. Furthermore, uninfected HBEpCs transfected with miR-4776 mimic showed decreased expression of NFKBIB mRNA. Overexpression of NFKBIB protein in IAV infected cells led to lower levels of IAV. Taken together, our data suggest that miRNA-4776 modulates IAV production in infected cells through NFKBIB expression, possibly through the modulation of NF-κB. View Full-Text

Keywords: NFKBIB; influenza virus; bronchial epithelial cells; NF-κB; virus survival NFKBIB; influenza virus; bronchial epithelial cells; NF-κB; virus survival





Autor: Sreekumar Othumpangat 1,* , Nicole B. Bryan 2, Donald H. Beezhold 1 and John D. Noti 1

Fuente: http://mdpi.com/



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