Characterization of Bacillus subtilis Viruses vB BsuM-Goe2 and vB BsuM-Goe3Reportar como inadecuado


Characterization of Bacillus subtilis Viruses vB BsuM-Goe2 and vB BsuM-Goe3


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1

Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, University of Goettingen, 37077, Goettingen, Germany

2

Department of General Microbiology, Institute of Microbiology and Genetics, University of Goettingen, 37077 Goettingen, Germany





*

Author to whom correspondence should be addressed.



Academic Editor: Laurent Debarbieux

Abstract The Spounavirinae viruses are ubiquitous in nature and have an obligatory virulent lifestyle. They infect Firmicutes, a bacterial phylum containing an array of environmental non-pathogenic and pathogenic organisms. To expand the knowledge of this viral subfamily, new strains were isolated and investigated in this study. Here we present two new viruses, vB BsuM-Goe2 and vB BsuM-Goe3, isolated from raw sewage and infecting Bacillus species. Both were morphologically classified via transmission electron microscopy TEM as members of the Spounavirinae subfamily belonging to the Myoviridae family. Genomic sequencing and analyses allowed further affiliation of vB BsuM-Goe2 to the SPO1-like virus group and vB BsuM-Goe3 to the Bastille-like virus group. Experimentally determined adsorption constant, latency period, burst size and host range for both viruses revealed different survival strategies. Thus vB BsuM-Goe2 seemed to rely on fewer host species compared to vB BsuM-Goe3, but efficiently recruits those. Stability tests pointed out that both viruses are best preserved in LB-medium or TMK-buffer at 4 or 21 °C, whereas cryopreservation strongly reduced viability. View Full-Text

Keywords: phage; virus; Bacillus subtilis; vB BsuM-Goe2; vB BsuM-Goe3; Spo1virus; Bastillevirus; Spounavirinae phage; virus; Bacillus subtilis; vB BsuM-Goe2; vB BsuM-Goe3; Spo1virus; Bastillevirus; Spounavirinae





Autor: Inka M. Willms 1, Michael Hoppert 2 and Robert Hertel 1,*

Fuente: http://mdpi.com/



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