Development and Evaluation of Monoclonal Antibodies for the Glucoside of T-2 Toxin T2-GlcReportar como inadecuado




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1

Bacterial Foodborne Pathogens and Mycology Research Unit, USDA-ARS-NCAUR, 1815 N. University St., Peoria, IL 61604, USA

2

Renewable Product Technology Research Unit, USDA-ARS-NCAUR, 1815 N. University St., Peoria, IL 61604, USA





*

Author to whom correspondence should be addressed.



Abstract The interactions between fungi and plants can yield metabolites that are toxic in animal systems. Certain fungi are known to produce sesquiterpenoid trichothecenes, such as T-2 toxin, that are biotransformed by several mechanisms including glucosylation. The glucosylated forms have been found in grain and are of interest as potential reservoirs of T-2 toxin that are not detected by many analytical methods. Hence the glucosides of trichothecenes are often termed -masked- mycotoxins. The glucoside of T-2 toxin T2-Glc was linked to keyhole limpet hemocyanin and used to produce antibodies in mice. Ten monoclonal antibody Mab-producing hybridoma cell lines were developed. The Mabs were used in immunoassays to detect T2-Glc and T-2 toxin, with midpoints of inhibition curves IC50s in the low ng-mL range. Most of the Mabs demonstrated good cross-reactivity to T-2 toxin, with lower recognition of HT-2 toxin. One of the clones 2-13 was further characterized with in-depth cross-reactivity and solvent tolerance studies. Results suggest Mab 2-13 will be useful for the simultaneous detection of T-2 toxin and T2-Glc. View Full-Text

Keywords: T-2 toxin; masked mycotoxins; metabolites; antibody; immunoassay T-2 toxin; masked mycotoxins; metabolites; antibody; immunoassay





Autor: Chris M. Maragos 1,* , Cletus Kurtzman 1, Mark Busman 1, Neil Price 2 and Susan McCormick 1

Fuente: http://mdpi.com/



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