Quantitative Mass Spectrometric Analysis and Post-Extraction Stability Assessment of the Euglenoid Toxin EuglenophycinReportar como inadecuado




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1

Center for Coastal Studies Texas A&M University Corpus Christi 6300 Ocean Drive Corpus Christi, TX 78412, USA

2

Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA

3

NOAA-NCCOS Center for Human Health Research Hollings Marine Laboratory, 331 Fort Johnson Rd, Charleston, SC 29412, USA

4

Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA





*

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Abstract Euglenophycin is a recently discovered toxin produced by at least one species of euglenoid algae. The toxin has been responsible for several fish mortality events. To facilitate the identification and monitoring of euglenophycin in freshwater ponds, we have developed a specific mass spectrometric method for the identification and quantitation of euglenophycin. The post-extraction stability of the toxin was assessed under various conditions. Euglenophycin was most stable at room temperature. At 8 °C there was a small, but statistically significant, loss in toxin after one day. These methods and knowledge of the toxin’s stability will facilitate identification of the toxin as a causative agent in fish kills and determination of the toxin’s distribution in the organs of exposed fish. View Full-Text

Keywords: euglena; euglenophycin; stability; toxin euglena; euglenophycin; stability; toxin





Autor: Danielle B. Gutierrez 1, Alexandra Rafalski 2, Kevin Beauchesne 3, Peter D. Moeller 3, Richard E. Triemer 4 and Paul V. Zimba 1,*

Fuente: http://mdpi.com/



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