An Amperometric Biosensor for Uric Acid Determination Prepared From Uricase Immobilized in Polyaniline-Polypyrrole FilmReportar como inadecuado




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Department of Chemistry, Faculty of Arts and Sciences, Gazi University, 06500 Ankara, Turkey





Abstract A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole pani-ppy composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag-AgCl. The linear working range of the biosensor was 2.5×10-6 – 8.5×10-5 M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 oC, respectively. The stability and reproducibility of the enzyme electrode have been also studied. View Full-Text

Keywords: Uric acid; Uricase; Biosensor; Polypyrrole; Polyaniline Uric acid; Uricase; Biosensor; Polypyrrole; Polyaniline





Autor: Fatma Arslan

Fuente: http://mdpi.com/



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