Use of Fe3O4 Nanoparticles for Enhancement of Biosensor Response to the Herbicide 2,4-Dichlorophenoxyacetic AcidReportar como inadecuado




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1

School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia, Bangi, 43000, Selangor, Malaysia

2

Biotechnology Research Centre, Malaysian Agriculture Research & Development Institute MARDI, P.O. BOX 12301, GPO Kuala Lumpur, Malaysia





*

Author to whom correspondence should be addressed.



Abstract Magnetic nanoparticles of Fe3O4 were synthesized and characterized using transmission electron microscopy and X-ray diffraction. The Fe3O4 nanoparticles were found to have an average diameter of 5.48 ±1.37 nm. An electrochemical biosensor based on immobilized alkaline phosphatase ALP and Fe3O4 nanoparticles was studied. The amperometric biosensor was based on the reaction of ALP with the substrate ascorbic acid 2-phosphate AA2P. The incorporation of the Fe3O4 nanoparticles together with ALP into a sol gel-chitosan biosensor membrane has led to the enhancement of the biosensor response, with an improved linear response range to the substrate AA2P 5-120 μM and increased sensitivity. Using the inhibition property of the ALP, the biosensor was applied to the determination of the herbicide 2,4-dichlorophenoxyacetic acid 2,4-D. The use of Fe3O4 nanoparticles gives a two-fold improvement in the sensitivity towards 2,4-D, with a linear response range of 0.5-30 μgL-1. Exposure of the biosensor to other toxicants such as heavy metals demonstrated only slight interference from metals such as Hg2+, Cu2+, Ag2+ and Pb2+. The biosensor was shown to be useful for the determination of the herbicide 2, 4-D because good recovery of 95-100 percent was obtained, even though the analysis was performed in water samples with a complex matrix. Furthermore, the results from the analysis of 2,4-D in water samples using the biosensor correlated well with a HPLC method. View Full-Text

Keywords: Screen-printer biosensor; alkaline phosphatase; Fe3O4 nanoparticles; 2; 4- dichlorophenoxyacetic acid Screen-printer biosensor; alkaline phosphatase; Fe3O4 nanoparticles; 2; 4- dichlorophenoxyacetic acid





Autor: Kee-Shyuan Loh 1, Yook Heng Lee 1,* , Ahmad Musa 1, Abdul Aziz Salmah 2 and Ishak Zamri 2

Fuente: http://mdpi.com/



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