Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like ApproachReportar como inadecuado




Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Biosensors and Bioanalysis Group, FCEN, UBA - Department of Biochemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, 1428 Buenos Aires, Argentina

2

Biosensors and Bioanalysis Group, FCEN, UBA and CONICET - Department of Biochemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. 2, 1428 Buenos Aires, Argentina





*

Author to whom correspondence should be addressed.



Abstract Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol-1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed. View Full-Text

Keywords: Biosensors; microbial metabolism; Arrhenius plot; activation energy; Saccharomyces cerevisiae; glucose transport Biosensors; microbial metabolism; Arrhenius plot; activation energy; Saccharomyces cerevisiae; glucose transport





Autor: Diego J. Kormes 1 and Eduardo Cortón 2,*

Fuente: http://mdpi.com/



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