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1

Department of Chemistry, Vanderbilt University, VU Station B. Nashville, TN 37235, USA

2

Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, VU Station B. Nashville, TN 37235. USA

3

Edgewood Chemical and Biological Center, Aberdeen Proving Ground, MD 21010. USA





*

Author to whom correspondence should be addressed.



Abstract Harnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer MMP that is capable of simultaneously measuring flux changes in four extracellular analytes acidification rate, glucose uptake, oxygen uptake, and lactate production in real-time. Differential short-term cellular responses were observed between botulinum neurotoxin A and ricin toxin with neuroblastoma cells, alamethicin and anthrax protective antigen with RAW macrophages, and cholera toxin, muscarine, 2,4-dinitro-phenol, and NaF with CHO cells. These results and the post exposure dynamics and metabolic recovery observed in each case suggest the usefulness of cell-based detectors to discriminate between specific analytes and classes of compounds in a complex matrix, and furthermore to make metabolic inferences on the cellular effects of the agents. This may be particularly valuable for classifying unknown toxins. View Full-Text

Keywords: toxin; biotoxin; biosensors; microphysiometry toxin; biotoxin; biosensors; microphysiometry





Autor: Sven E. Eklund 1, 2, Roy G. Thompson 3, Rachel M. Snider 1, Clare K. Carney 1, David W. Wright 1, John Wikswo 2 and David E. Cliffel 1, 2,*

Fuente: http://mdpi.com/



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