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1

CNR – IMM Catania, Italy

2

Università degli Studi di Catania, Dipartimento di Chimica Biologica, Chimica Medica e Biologia Molecolare, Catania, Italy

3

Università degli Studi di Catania, Dipartimento di Scienze Biomediche, Catania, Italy

4

Laboratorio Superfici e Interfasi SUPERLAB, Consorzio Catania Ricerche, Catania, Italy





*

Author to whom correspondence should be addressed.



Abstract The aim of this paperis to summarize the efforts carried out so far in the fabrication of Si-based biosensors by a team of researchers in Catania, Italy. This work was born as a collaboration between the Catania section of the Microelectronic and Microsystem Institute IMM of the CNR, the Surfaces and Interfaces laboratory SUPERLAB of the Consorzio Catania Ricerche and two departments at the University of Catania: the Biomedical Science and the Biological Chemistry and Molecular Biology Departments. The first goal of our study was the definition and optimization of an immobilization protocol capable of bonding the biological sensing element on a Si-based surface via covalent chemical bonds. We chose SiO2 as the anchoring surface due to its biocompatibility and extensive presence in microelectronic devices. The immobilization protocol was tested and optimized, introducing a new step, oxide activation, using techniques compatible with microelectronic processing. The importance of the added step is described by the experimental results. We also tested different biological molecule concentrations in the immobilization solutions and the effects on the immobilized layer. Finally a MOS-like structure was designed and fabricated to test an electrical transduction mechanism. The results obtained so far and the possible evolution of the research field are described in this review paper. View Full-Text

Keywords: Si-based biosensors; biological molecules immobilization; glutaraldehyde; glucose oxidase; DNA strands; metallothioneines; microelectronic compatibility Si-based biosensors; biological molecules immobilization; glutaraldehyde; glucose oxidase; DNA strands; metallothioneines; microelectronic compatibility





Author: Sebania Libertino 1,* , Venera Aiello 2, 3, Antonino Scandurra 4, Marcella Renis 2, Fulvia Sinatra 3 and Salvatore Lombardo 1

Source: http://mdpi.com/



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