Micro-Pattern Guided Adhesion of Osteoblasts on Diamond SurfacesReportar como inadecuado




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1

Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 6, Czech Republic

2

Faculty of Electrical Engineering and Information Technology, Slovak University of Technology, Ilkovičova 3, Bratislava, Slovak Republic

3

Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University, Ke Karlovu 2, Prague 2, Czech Republic





*

Authors to whom correspondence should be addressed.



Abstract Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells 2,500-10,000 cells-cm2and fetal bovine serum 0-15%. The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy AFM. The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase imagesindicate different conformation of the proteins and explain the cell selectivity. View Full-Text

Keywords: cell adhesion; proteins; diamond; atomic force microscopy; biotechnology; tissue engineering; biosensors; osteoblasts cell adhesion; proteins; diamond; atomic force microscopy; biotechnology; tissue engineering; biosensors; osteoblasts





Autor: Bohuslav Rezek 1,* , Lenka Michalíková 1, 2,* , Egor Ukraintsev 1, Alexander Kromka 1 and Marie Kalbacova 3

Fuente: http://mdpi.com/



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