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1

Department of Biomedical Engineering - Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA

2

College of Nanoscale Science and Engineering - University at Albany State University of New York, 255 Fuller Rd., Albany, NY 12203, USA

3

Department of Food Science - Cornell University, 312 Stocking Hall, Ithaca, NY 14853, USA





*

Author to whom correspondence should be addressed.



Abstract The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform. View Full-Text

Keywords: pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-chip pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-chip





Autor: Clarissa Lui 1,* , Nathaniel C. Cady 2 and Carl A. Batt 3

Fuente: http://mdpi.com/



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