Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in FoodReport as inadecuate




Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food - Download this document for free, or read online. Document in PDF available to download.

1

Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA

2

School of Aeronautics & Astronautics, Purdue University, 701 W. Stadium Ave., West Lafayette, Indiana 47907, USA

3

United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA





*

Author to whom correspondence should be addressed.



Abstract Salmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence TRF assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody MAb 2F-11 was used as the reporter. Detection occurred when an evanescent wave from a laser 635 nm excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu-mL in pure culture and 104 cfu-mL with egg and chicken breast samples when spiked with 102 cfu-mL after 2–6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods. View Full-Text

Keywords: Salmonella; fiber optic sensor; time–resolved fluorescence assay; egg; chicken Salmonella; fiber optic sensor; time–resolved fluorescence assay; egg; chicken





Author: Angela M. Valadez 1, Carlos A. Lana 2, Shu-I Tu 3, Mark T. Morgan 1 and Arun K. Bhunia 1,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents