Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4-Chitosan Core-Shell MicrospheresReport as inadecuate




Amperometric Hydrogen Peroxide Biosensor Based on Immobilization of Hemoglobin on a Glassy Carbon Electrode Modified with Fe3O4-Chitosan Core-Shell Microspheres - Download this document for free, or read online. Document in PDF available to download.

College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China





*

Author to whom correspondence should be addressed.



Abstract Novel magnetic Fe3O4-chitosan CS microspheres were prepared using magnetic Fe3O4 nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin Hb immobilization method, an innovative biosensor with a Fe3O4-CS-Hb-Fe3O4-CS -sandwich- configuration was constructed. This biosensor had a fast less than 10 s response to H2O2 and excellent linear relationships were obtained in the concentration range of 5.0 × 10-5 to 1.8 × 10-3 M and 1.8 × 10-3 to 6.8 × 10-3 M with a detection limit of 4.0 × 10-6 M s-n = 3 under the optimum conditions. The apparent Michaelis-Menten constant Km was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H2O2 concentration in real samples. View Full-Text

Keywords: hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone hemoglobin; hydrogen peroxide; magnetic microspheres; biosensor; hydroquinone





Author: Xue-Cai Tan * , Jin-Lei Zhang, Sheng-Wei Tan, Dan-Dan Zhao, Zen-Wei Huang, Yan Mi and Zai-Yin Huang

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents