The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step ApproachReportar como inadecuado




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1

Department of Biology, Brown University, 171 Meeting Street, Providence, RI 02912, USA

2

College of Medicine, University of Cincinnati, 231 Albert B. Sabin Way, Cincinnati, OH 45267, USA

3

Department of Chemical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA





*

Author to whom correspondence should be addressed.



Abstract A one-step, single use, disposable Alkaline Phosphatase ALP biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU-L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM-L, 5 mg-L and 400 mg-L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding. View Full-Text

Keywords: alkaline phosphatase; electrochemical detection; biosensor alkaline phosphatase; electrochemical detection; biosensor





Autor: Joanne H. Wang 1, Kevin Wang 2, Brandon Bartling 3 and Chung-Chiun Liu 3,*

Fuente: http://mdpi.com/



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