Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe-CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer CellsReport as inadecuate




Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe-CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cells - Download this document for free, or read online. Document in PDF available to download.

1

WPI Immunology Frontier Research Center, Osaka University, Yamada-oka 1-3, Suita, Osaka 565-0871, Japan

2

Graduate School of Frontier Biosciences, Osaka University, Yamada-oka 2-1, Suita, Osaka 565-0871, Japan





*

Author to whom correspondence should be addressed.



Abstract The early detection of HER2 human epidermal growth factor receptor 2 status in breast cancer patients is very important for the effective implementation of anti-HER2 antibody therapy. Recently, HER2 detections using antibody conjugated quantum dots QDs have attracted much attention. QDs are a new class of fluorescent materials that have superior properties such as high brightness, high resistance to photo-bleaching, and multi-colored emission by a single-light source excitation. In this study, we synthesized three types of anti-HER2 antibody conjugated QDs HER2Ab-QDs using different coupling agents EDC-sulfo-NHS, iminothiolane-sulfo-SMCC, and sulfo-SMCC. As water-soluble QDs for the conjugation of antibody, we used glutathione coated CdSe-CdZnS QDs GSH-QDs with fluorescence quantum yields of 0.23~0.39 in aqueous solution. Dispersibility, hydrodynamic size, and apparent molecular weights of the GSH-QDs and HER2Ab-QDs were characterized by using dynamic light scattering, fluorescence correlation spectroscopy, atomic force microscope, and size-exclusion HPLC. Fluorescence imaging of HER2 overexpressing cells KPL-4 human breast cancer cell line was performed by using HER2Ab-QDs as fluorescent probes. We found that the HER2Ab-QD prepared by using SMCC coupling with partially reduced antibody is a most effective probe for the detection of HER2 expression in KPL-4 cells. We have also studied the size dependency of HER2Ab-QDs with green, orange, and red emission on the fluorescence image of KPL-4 cells. View Full-Text

Keywords: quantum dots; HER2; antibody; cellular imaging; breast cancer cell quantum dots; HER2; antibody; cellular imaging; breast cancer cell





Author: Dhermendra K. Tiwari 1, Shin-Ichi Tanaka 2, Yasushi Inouye 2, Keiko Yoshizawa 1, Tomonobu M. Watanabe 1 and Takashi Jin 1,2,*

Source: http://mdpi.com/



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