Flow Injection Chemiluminescent Immunoassay for Carcinoembryonic Antigen Using Boronic Immunoaffinity ColumnReportar como inadecuado




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1

Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, 210004, China

2

Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210097, China





*

Author to whom correspondence should be addressed.



Abstract A flow injection chemiluminescence immunoassay for rapid and sensitive detection of carcinoembryonic antigen CEA by using a phenylboronic acid-based immunoaffinity column as a glycoprotein collector was proposed in this paper. The column was prepared by coupling of 3-aminophenylboronic acid on the glass beads through a γ-glycidoxypropyltrimethoxysilane GPMS linkage. Based on an indirect competitive immunoreaction, the mixture of CEA sample and enzyme conjugated CEA antibody HRP-anti-CEA was incubated in advance, followed by direct injection to the column to capture free HRP-labeled CEA antibody in the column. The trapped HRP-labeled antibody was detected by flow inject chemiluminescence in the presence of luminol and hydrogen peroxide. The decreased chemiluminescent signal was proportional to the concentration of CEA in the range of 3.0–30.0 ng-mL with a correlation coefficient of 0.998. The column showed an acceptable reproducibility and stability and is potentially used for practical clinical detection of the serum CEA level. View Full-Text

Keywords: flow injection chemiluminescence immunoassay; carcinoembryonic antigen; boronic immunoaffinity column flow injection chemiluminescence immunoassay; carcinoembryonic antigen; boronic immunoaffinity column





Autor: Xiaoling Ma 1, Huihui Li 2, Min Wu 2, Chong Sun 2, Laifa Li 2 and Xiaodi Yang 2,*

Fuente: http://mdpi.com/



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