1-Hydroxypyrene–A Biochemical Marker for PAH Pollution Assessment of Aquatic EcosystemReportar como inadecuado




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1

Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 612 42, Brno, Czech Republic

2

Institute of Public Health Ostrava, Partyzanske namesti 7, 702 00 Ostrava, Czech Republic

3

Czech Hydrometeorological Institute, Kroftova 43, 616 67, Brno, Czech Republic





*

Author to whom correspondence should be addressed.



Abstract The aim of the present study was to assess aquatic contamination by polycyclic aromatic hydrocarbons PAH, using the 1-hydroxypyrene 1-OHP content in fish bile as a biochemical marker. A total of 71 chub Leuciscus cephalus L. were collected from seven locations on the Svitava and Svratka rivers in and around the industrial city of Brno, Czech Republic. The levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection after deconjugation. Normalising the molar concentration of the biliary 1-OHP to the biliary protein content reduced sample variation. The content of 1-OHP was correlated with the PAH level in bottom sediment and semi-permeable membrane devices SPMD, which was analyzed by a combination of HPLC-FLD and GC-MS methods. The highest mean values of 1-OHP were found in fish caught at the Svratka River at locations Modrice 169.2 ± 99.7 ng•mg-1 protein and Rajhradice 152.2 ± 79.7 ng•mg-1 protein, which are located downstream from Brno. These values were significantly P 0.05 higher than those obtained from localities Knínicky 98.4 ± 66.1 ng•mg-1 protein and Bílovice nad Svitavou 64.1 ± 31.4 ng•mg-1 protein. The lowest contents of PAH in sediment and SPMD were found at location Knínicky 1.5 mg•kg-1 dry mass and 19.4 ng•L-1, respectively. The highest contents of PAH in sediment and SPMD were found in Rajhradice 26.0 mg•kg-1 dry mass and Svitava before junction 65.4 ng•L-1, respectively. A Spearman correlation test was applied to determine the relationship between biliary 1-OHP and the sum of PAH in sediment and SPMD. A positive, but no statistically significant correlation was found. The main impact sources of elevated level of PAHs in sites located downstream from Brno are most probably intensive industrial and agricultural activities and domestic waste. View Full-Text

Keywords: aquatic pollution; fish; bile; SPMD; the Svitava and Svratka rivers; polycyclic aromatic hydrocarbons aquatic pollution; fish; bile; SPMD; the Svitava and Svratka rivers; polycyclic aromatic hydrocarbons





Autor: Jana Blahova 1,* , Kamila Kruzikova 1, Barbora Kasiková 2, Pavel Stierand 3, Jana Jurcikova 2, Tomas Ocelka 2 and Zdenka Svobodova 1

Fuente: http://mdpi.com/



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