Fluorescence-based Sensing of 2,4,6-Trinitrotoluene TNT Using a Multi-channeled Polymethyl methacrylate PMMA MicroimmunosensorReportar como inadecuado




Fluorescence-based Sensing of 2,4,6-Trinitrotoluene TNT Using a Multi-channeled Polymethyl methacrylate PMMA Microimmunosensor - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Center for Bio-Molecular Science and Engineering Code 6920, US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA





*

Author to whom correspondence should be addressed.



Abstract Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene TNT were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly methyl methacrylate PMMA via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene AlexaFluor-Cad-TNB was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng-mL ppb with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine RDX, HMX, 2,4-dinitrotoluene DNT, 4-nitrotoluene 4-NT and 2-amino-4,6-DNT. View Full-Text

Keywords: TNT; immunosensor; fluorescence; PMMA; microchannel; antibody TNT; immunosensor; fluorescence; PMMA; microchannel; antibody





Autor: Paul T. Charles * , Andre A. Adams, Peter B. Howell, Scott A. Trammell, Jeffrey R. Deschamps and Anne W. Kusterbeck

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados