Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of Amyloid-β PeptideReportar como inadecuado




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Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10 Street, 10-747 Olsztyn, Poland





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Abstract The main objective of the presented study was the development of a simple analytical tool for exploring the influence of naturally occurring compounds on the aggregation of amyloid-β peptide Aβ40 in order to find potential anti-neurodegenerative drugs. The gold discs used for surface plasmon resonance SPR measurements were modified with thioaliphatic acid. The surface functionalized with carboxylic groups was used for covalent attaching of Aβ40 probe by creation of amide bonds in the presence of EDC-NHS. The modified SPR gold discs were used for exploring the Aβ40 aggregation process in the presence of selected alkaloids: arecoline hydrobromide, pseudopelletierine hydrochloride, trigonelline hydrochloride and α-lobeline hydrochloride. The obtained results were discussed with other parameters which govern the phenomenon studied such as lipophilicity- hydrophilicy and Aβ40-alkaloid association constants. View Full-Text

Keywords: amyloid-β peptide; surface plasmon resonance; alkaloids; thioaliphatic acid monolayer amyloid-β peptide; surface plasmon resonance; alkaloids; thioaliphatic acid monolayer





Autor: Bartłomiej Emil Kraziński, Jerzy Radecki and Hanna Radecka *

Fuente: http://mdpi.com/



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