Detection of Prostate Stem Cell Antigen Expression in Human Prostate Cancer Using Quantum-Dot-Based TechnologyReportar como inadecuado




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1

Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China

2

Department of Urology, Angers Teaching Hospital, 4, Rue Larrey, Angers 49000, France





*

Author to whom correspondence should be addressed.



Abstract Quantum dots QDs are a new class of fluorescent labeling for biological and biomedical applications. In this study, we detected prostate stem cell antigen PSCA expression correlated with tumor grade and stage in human prostate cancer by QDs-based immunolabeling and conventional immunohistochemistry IHC, and evaluated the sensitivity and stability of QDs-based immunolabeling in comparison with IHC. Our data revealed that increasing levels of PSCA expression accompanied advanced tumor grade QDs labeling, r = 0.732, p 0.001; IHC, r = 0.683, p 0.001 and stage QDs labeling, r = 0.514, p = 0.001; IHC, r = 0.432, p = 0.005, and the similar tendency was detected by the two methods. In addition, by comparison between the two methods, QDs labeling was consistent with IHC in detecting the expression of PSCA in human prostate tissue correlated with different pathological types K = 0.845, p 0.001. During the observation time, QDs exhibited superior stability. The intensity of QDs fluorescence remained stable for two weeks p = 0.083 after conjugation to the PSCA protein, and nearly 93% of positive expression with their fluorescence still could be seen after four weeks. View Full-Text

Keywords: prostate stem cell antigen; prostate cancer; quantum dots; fluorescence prostate stem cell antigen; prostate cancer; quantum dots; fluorescence





Autor: Yuan Ruan 1, Weimin Yu 1, Fan Cheng 1,* , Xiaobin Zhang 1 and Stéphane Larré 2

Fuente: http://mdpi.com/



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