Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from CompostReportar como inadecuado




Quorum Sensing Activity of Aeromonas Caviae Strain YL12, A Bacterium Isolated from Compost - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia





*

Author to whom correspondence should be addressed.



Abstract Quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. Isolate YL12 was identified using matrix-assisted laser desorption ionization time-of-flight MALDI-TOF mass spectrometry and molecular typing using rpoD gene which identified the isolate as Aeromonas caviae. High resolution tandem mass spectrometry was subsequently employed to identify the N-acyl homoserine lactone profile of Aeromonas caviae YL12 and confirmed that this isolate produced two short chain N-acyl homoserine lactones, namely C4-HSL and C6, and the production was observed to be cell density-dependent. Using the thin layer chromatography TLC bioassay, both AHLs were found to activate C. violaceum CV026, whereas only C6-HSL was revealed to induce bioluminescence expression of E. coli pSB401. The data presented in this study will be the leading steps in understanding the role of quorum sensing in Aeromonas caviae strain YL12. View Full-Text

Keywords: Cell-to-cell communication; C4-HSL N-butyryl-L-homoserine lactone; C6-HSL N-hexanoyl-L-homoserine lactone; N-acyl homoserine lactone AHL; MALDI-TOF; triple quodruopole liquid chromatography mass spectrometry LCMS-MS; Pacific Biosciences; RpoD; thin liquid chromatography TLC Cell-to-cell communication; C4-HSL N-butyryl-L-homoserine lactone; C6-HSL N-hexanoyl-L-homoserine lactone; N-acyl homoserine lactone AHL; MALDI-TOF; triple quodruopole liquid chromatography mass spectrometry LCMS-MS; Pacific Biosciences; RpoD; thin liquid chromatography TLC





Autor: Yan-Lue Lim, Robson Ee, Wai-Fong Yin and Kok-Gan Chan *

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados