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1

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China

2

Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200000, China

3

Yangtze Delta Region Institute of Tsinghua University, Jiaxing 310013, China





*

Author to whom correspondence should be addressed.



Abstract A colloidal gold immunochromatographic assay GICA was developed for rapid detection of chloramphenicol CAP residues in aquatic products. A nitrocellulose NC membrane was used as the carrier, and the polyclonal CAP antibody was used as the marker protein. The average diameter of as-prepared colloidal gold nanoparticles AuNPs was about 20 nm. The optimal pH value of colloidal gold solutions and the amount of the antibody of CAP were 8.0 and 7.2 μg-mL, respectively. The CAP antibody was immobilized onto the conjugate pad after purification. The CAP conjugate and goat anti-rabbit IgG secondary antibody were coated onto the NC membrane. Next, the non-specific sites were blocked with 1% bovine serum albumin. The minimum detectable concentration of CAP in standard solution is 0.5 ng-mL, with good reproducibility. For the real samples from crucian carps injected with a single-dose of CAP in the dorsal muscles, the minimum detectable concentration of CAP residues was 0.5 µg-kg. The chromatographic analysis time was less than 10 min, and the strip had a long storage lifetime of more than 90 days at different temperatures. The strips provide a means for rapid detection of CAP residues in aquatic products. View Full-Text

Keywords: chloramphenicol; colloidal gold nanoparticles; fishery drug residues; immunochromatographic assay chloramphenicol; colloidal gold nanoparticles; fishery drug residues; immunochromatographic assay





Autor: Chennan Zhou 1, Xueyin Zhang 1, Xinxin Huang 2, Xishan Guo 1,* , Qiang Cai 3 and Songming Zhu 1

Fuente: http://mdpi.com/



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