Evaluation of Optical Detection Platforms for Multiplexed Detection of Proteins and the Need for Point-of-Care Biosensors for Clinical UseReportar como inadecuado




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Division of Biology, Chemistry, and Materials Science Office of Science and Engineering Laboratories; U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA





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Abstract This review investigates optical sensor platforms for protein multiplexing, the ability to analyze multiple analytes simultaneously. Multiplexing is becoming increasingly important for clinical needs because disease and therapeutic response often involve the interplay between a variety of complex biological networks encompassing multiple, rather than single, proteins. Multiplexing is generally achieved through one of two routes, either through spatial separation on a surface different wells or spots or with the use of unique identifiers-labels such as spectral separation—different colored dyes, or unique beads—size or color. The strengths and weaknesses of conventional platforms such as immunoassays and new platforms involving protein arrays and lab-on-a-chip technology, including commercially-available devices, are discussed. Three major public health concerns are identified whereby detecting medically-relevant markers using Point-of-Care POC multiplex assays could potentially allow for a more efficient diagnosis and treatment of diseases. View Full-Text

Keywords: immunoassay; fluorescent; ELISA; multiplex; protein; biomarker; point-of-care; in vitro diagnostics; quantum dot immunoassay; fluorescent; ELISA; multiplex; protein; biomarker; point-of-care; in vitro diagnostics; quantum dot





Autor: Samantha Spindel * and Kim E. Sapsford

Fuente: http://mdpi.com/



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