An Optical Biosensor from Green Fluorescent Escherichia coli for the Evaluation of Single and Combined Heavy Metal ToxicitiesReportar como inadecuado




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1

School of Chemical Science and Food Technolgoy, Faculty of Science, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia

2

Southeast Asia Disaster Prevention Research Initiative SEADPRI-UKM, LESTARI, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D.E., Malaysia





*

Author to whom correspondence should be addressed.



Academic Editor: M. Selim Ünlü

Abstract A fluorescence-based fiber optic toxicity biosensor based on genetically modified Escherichia coli E. coli with green fluorescent protein GFP was developed for the evaluation of the toxicity of several hazardous heavy metal ions. The toxic metals include CuII, CdII, PbII, ZnII, CrVI, CoII, NiII, AgI and FeIII. The optimum fluorescence excitation and emission wavelengths of the optical biosensor were 400 ± 2 nm and 485 ± 2 nm, respectively. Based on the toxicity observed under optimal conditions, the detection limits of CuII, CdII, PbII, ZnII, CrVI, CoII, NiII, AgI and FeIII that can be detected using the toxicity biosensor were at 0.04, 0.32, 0.46, 2.80, 100, 250, 400, 720 and 2600 μg-L, respectively. The repeatability and reproducibility of the proposed biosensor were 3.5%–4.8% RSD relative standard deviation and 3.6%–5.1% RSD n = 8, respectively. The biosensor response was stable for at least five weeks, and demonstrated higher sensitivity towards metal toxicity evaluation when compared to a conventional Microtox assay. View Full-Text

Keywords: whole cell biosensor; hazardous heavy metal; optical fiber biosensor; GFP E. coli whole cell biosensor; hazardous heavy metal; optical fiber biosensor; GFP E. coli





Autor: Dedi Futra 1, Lee Yook Heng 1,2,* , Asmat Ahmad 1, Salmijah Surif 1 and Tan Ling Ling 2

Fuente: http://mdpi.com/



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