Centrifugal Microfluidic System for Nucleic Acid Amplification and DetectionReportar como inadecuado




Centrifugal Microfluidic System for Nucleic Acid Amplification and Detection - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

State Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong University, Xi’an 710054, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editors: Zhiping Wang and Charles Chun Yang

Abstract We report here the development of a rapid PCR microfluidic system comprising a double-shaft turntable and centrifugal-based disc that rapidly drives the PCR mixture between chambers set at different temperatures, and the bidirectional flow improved the space utilization of the disc. Three heating resistors and thermistors maintained uniform, specific temperatures for the denaturation, annealing, and extension steps of the PCR. Infrared imaging showed that there was little thermal interference between reaction chambers; the system enabled the cycle number and reaction time of each step to be independently adjusted. To validate the function and efficiency of the centrifugal microfluidic system, a 350-base pair target gene from the hepatitis B virus was amplified and quantitated by fluorescence detection. By optimizing the cycling parameters, the reaction time was reduced to 32 min as compared to 120 min for a commercial PCR machine. DNA samples with concentrations ranging from 10 to 106 copies-mL could be quantitatively analyzed using this system. This centrifugal-based microfluidic platform is a useful system and possesses industrialization potential that can be used for portable diagnostics. View Full-Text

Keywords: centrifugal microfluidics; PCR; real-time fluorescence detection; Lab-on-a-disc centrifugal microfluidics; PCR; real-time fluorescence detection; Lab-on-a-disc





Autor: Baogang Miao †, Niancai Peng * , Lei Li †, Zheng Li, Fei Hu, Zengming Zhang and Chaohui Wang

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados