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1

Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China

2

The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China

3

Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA





*

Author to whom correspondence should be addressed.



Academic Editor: Paul E. Laibinis

Abstract A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide GO for rapid and facile detection of adenosine triphosphate ATP, as a model target. The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand CPDNA that partially hybridizes to the DNA biosensor. Then, the polymerization-nicking enzyme synergetic isothermal amplification could be triggered, leading to the synthesis of massive DNA amplicons, thus achieving an enhanced sensitivity with a wide linear dynamic response range of four orders of magnitude and good selectivity. This biosensing strategy expands the applications of GO-DNA nanobiointerfaces in biological sensing, showing great potential in fundamental research and biomedical diagnosis. View Full-Text

Keywords: aptamer; DNA biosensor; graphene oxide; Isothermal amplification aptamer; DNA biosensor; graphene oxide; Isothermal amplification





Autor: Yu Mao 1,2, Yongli Chen 1,2, Song Li 1, Shuo Lin 1,3 and Yuyang Jiang 2,*

Fuente: http://mdpi.com/



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