A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme RecognitionReportar como inadecuado




A Stimuli-Responsive Biosensor of Glucose on Layer-by-Layer Films Assembled through Specific Lectin-Glycoenzyme Recognition - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China

2

State Key Laboratory Cultivation Base of Natural Gas Conversion, Ningxia University, Yinchuan 750021, China

3

School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China





*

Authors to whom correspondence should be addressed.



Academic Editor: Yu Lei

Abstract The research on intelligent bioelectrocatalysis based on stimuli-responsive materials or interfaces is of great significance for biosensors and other bioelectronic devices. In the present work, lectin protein concanavalin A Con A and glycoenzyme glucose oxidase GOD were assembled into {Con A-GOD}n layer-by-layer LbL films by taking advantage of the biospecific lectin-glycoenzyme affinity between them. These film electrodes possess stimuli-responsive properties toward electroactive probes such as ferrocenedicarboxylic acid FcCOOH2 by modulating the surrounding pH. The CV peak currents of FcCOOH2 were quite large at pH 4.0 but significantly suppressed at pH 8.0, demonstrating reversible stimuli-responsive on-off behavior. The mechanism of stimuli-responsive property of the films was explored by comparative experiments and attributed to the different electrostatic interaction between the films and the probes at different pH. This stimuli-responsive films could be used to realize active-inactive electrocatalytic oxidation of glucose by GOD in the films and mediated by FcCOOH2 in solution, which may establish a foundation for fabricating novel stimuli-responsive electrochemical biosensors based on bioelectrocatalysis with immobilized enzymes. View Full-Text

Keywords: stimuli-responsive biosensor; lectin-sugar specific interaction; layer-by-layer assembly; concanavalin A; enzyme immobilization; ferrocenedicarboxylic acid stimuli-responsive biosensor; lectin-sugar specific interaction; layer-by-layer assembly; concanavalin A; enzyme immobilization; ferrocenedicarboxylic acid





Autor: Huiqin Yao 1,* , Qianqian Gan 1, Juan Peng 2,3, Shan Huang 1, Meilin Zhu 1 and Keren Shi 2,*

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados