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1

CNR-ISPA, Institute of Sciences of Food Productions, via Monteroni km 7, 73100 Lecce, Italy

2

Biotecgen, c-o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Benoit Piro

Abstract Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to individuate a source of Salmonella contamination at early times of pre-enrichment culture. Forty mL of Salmonella spp. enrichment culture were processed by immunoseparation using the Pathatrix, as in AFNOR validated qPCR protocols. The Salmonella biosensor combined with immunoseparation showed a limit of detection of 100 bacteria-40 mL, with a 400 fold increase to previous results. qPCR analysis requires processing of bead-bound bacteria with lysis buffer and DNA clean up, with a limit of detection of 2 cfu-50 μL. Finally, a protein chip was developed and tested in screening and identification of 5 common pathogen species, Salmonella spp., E. coli, S. aureus, Campylobacter spp. and Listeria spp. The protein chip, with high specificity in species identification, is proposed to be integrated into a Lab-on-Chip system, for rapid and reproducible screening of Salmonella spp. and other pathogen species contaminating food productions. View Full-Text

Keywords: antibody; biosensors; detection; food pathogens; identification; labelling; Salmonella spp. antibody; biosensors; detection; food pathogens; identification; labelling; Salmonella spp.





Autor: Palmiro Poltronieri 1,†,* , Fabio Cimaglia 2,†, Enrico De Lorenzis 2, Maurizio Chiesa 2, Valeria Mezzolla 1 and Ida Barbara Reca 1

Fuente: http://mdpi.com/



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