A Sensitive and Label-Free PbII Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex-Thioflavin T ConformationReportar como inadecuado


A Sensitive and Label-Free PbII Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex-Thioflavin T Conformation


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Biometrology Laboratory, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China





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Academic Editor: Huangxian Ju

Abstract PbII can cause serious damaging effects to human health, and thus, the study of Pb2+ detection methods to sensitively and selectively monitor PbII pollution has significant importance. In this work, we have developed a label-free fluorescence sensing strategy based on a PbII DNAzyme cleavage and the ThT-G-quadruplex complex. In the presence of PbII, a G-rich tail was cut and released from the substrate strand, which then would form a G-quadruplex structure by combination with ThT dye. The fluorescence signal increase was then measured for sensitive PbII quantification with a limit of detection of 0.06 nM. Our sensor also demonstrated high selectivity against six different metal ions, which is very important for the analysis of complex samples. View Full-Text

Keywords: DNAzyme; heavy metal ions; lead ion; thioflavin T ThT DNAzyme; heavy metal ions; lead ion; thioflavin T ThT





Autor: Yanli Wen, Lele Wang, Lanying Li, Li Xu and Gang Liu *

Fuente: http://mdpi.com/



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