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Miniaturized Real-Time PCR on a Q3 System for Rapid KRAS Genotyping


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1

Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami, 18-95125 Catania, Italy

2

STMicroelectronics, Stradale Primosole, 50-95121 Catania, Italy





*

Authors to whom correspondence should be addressed.



Academic Editor: Alexander Star

Abstract Colorectal cancer CRC is an aggressive human malignancy with a complex genomic landscape harboring KRAS mutations. In 40%–60% of patients with CRC, constantly active KRAS proteins affect the prognosis, surgical strategy, and clinical benefit from therapy with anti-epidermal growth factor receptor EGFR agents. For this reason, there is a greater demand for minimally-invasive diagnostic devices to characterize the genetic pattern and prevent the acquired mechanism to drug resistance. The rapid developments in cutting-edge diagnostic techniques are expected to play a growing role in medicine and represent an attractive promise to identify potential responders to personalized medicine. Here we propose a new method to simultaneously detect the main KRAS mutations on the portable real-time PCR Q3 platform. This platform is based on hybrid silicon-plastic technology implemented in a miniaturized chip able to achieve a sample-in answer-out rapid analysis, allowing a new approach to genetic counseling and testing. View Full-Text

Keywords: qPCR; Genome-based test; diagnostics; colorectal cancer qPCR; Genome-based test; diagnostics; colorectal cancer





Autor: Maria Guarnaccia 1, Rosario Iemmolo 1, Salvatore Petralia 2, Sabrina Conoci 2,* and Sebastiano Cavallaro 1,*

Fuente: http://mdpi.com/



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