An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew Drosera rotundifolia L.Reportar como inadecuado




An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew Drosera rotundifolia L. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090, Vienna, Austria





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Abstract An efficient procedure for in vitro shoot production of the medicinal plant sundew Drosera rotundifolia L. was developed. Of three cytokinins tested, zeatin ZEA at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA step 1: shoot induction with a 5-weeks main culture without plant growth regulators step 2: shoot elongation. Liquid media were superior to semisolid media: An average of 27.4 shoots per leaf explant, and 53.3 shoots per shoot explant were achieved. The 2-step culture system with liquid media permits a comparatively cheap micropropagation of sundew as well as in vitro biomass production, with potential for scale-up.

Keywords: Endangered; Medicinal Plant; Liquid Medium; Micropropagation Endangered; Medicinal Plant; Liquid Medium; Micropropagation





Autor: Christoph WAWROSCH * , Eva BENDA and Brigitte KOPP

Fuente: http://mdpi.com/



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