Experimental and theoretical study of light scattering by individual mature red blood cells by use of scanning flow cytometry and discrete dipole approximation - Physics OpticsReportar como inadecuado




Experimental and theoretical study of light scattering by individual mature red blood cells by use of scanning flow cytometry and discrete dipole approximation - Physics Optics - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Abstract: Elastic light scattering by mature red blood cells RBCs was theoreticallyand experimentally analyzed with the discrete dipole approximation DDA andthe scanning flow cytometry SFC, respectively. SFC permits measurement ofangular dependence of light-scattering intensity indicatrix of singleparticles. A mature RBC is modeled as a biconcave disk in DDA simulations oflight scattering. We have studied the effect of RBC orientation related to thedirection of the incident light upon the indicatrix. Numerical calculations ofindicatrices for several aspect ratios and volumes of RBC have been carriedout. Comparison of the simulated indicatrices and indicatrices measured by SFCshowed good agreement, validating the biconcave disk model for a mature RBC. Wesimulated the light-scattering output signals from the SFC with the DDA forRBCs modeled as a disk-sphere and as an oblate spheroid. The biconcave disk,the disk-sphere, and the oblate spheroid models have been compared for twoorientations, i.e. face-on and rim-on incidence. Only the oblate spheroid modelfor rim-on incidence gives results similar to the rigorous biconcave diskmodel.



Autor: Maxim A. Yurkin, Konstantin A. Semyanov, Peter A. Tarasov, Andrei V. Chernyshev, Alfons G. Hoekstra, Valeri P. Maltsev

Fuente: https://arxiv.org/



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