Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic AcidReportar como inadecuado


Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic Acid


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Department of Forest Science, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi 321-8505, Japan



Current address: Forest Tree Breeding Center, Forestry and Forest Products Research Institute, 3809-1 Ishi, Juo, Hitachi, Ibaraki 319-1301, Japan.



The late professor of Utsunomiya University.





*

Author to whom correspondence should be addressed.



Abstract The present study was performed to unravel the mechanisms of systemic acquired resistance SAR establishment and resistance signaling pathways against the canker-rot fungus Inonotus obliquus strain IO-U1 infection in Japanese birch plantlet No.8. Modulation of protein-profile induced by salicylic acid SA-administration was analyzed, and SA-responsive proteins were identified. In total, 5 specifically expressed, 3 significantly increased, and 3 significantly decreased protein spots were identified using liquid chromatography-tandem mass spectrometry LC-MS-MS and the sequence tag method. These proteins were malate dehydrogenase, succinate dehydrogenase, phosphoglycerate kinase, diaminopimalate decarboxylase, arginase, chorismate mutase, cyclophilin, aminopeptidase, and unknown function proteins. These proteins are considered to be involved in SAR-establishment mechanisms in the Japanese birch plantlet No 8. View Full-Text

Keywords: Japanese birch; Inonotus obliquus; salicylic acid Japanese birch; Inonotus obliquus; salicylic acid





Autor: Hiromu Suzuki, Yuya Takashima †, Futoshi Ishiguri, Nobuo Yoshizawa ‡ and Shinso Yokota *

Fuente: http://mdpi.com/



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