Magnetically-Responsive Hydrogels for Modulation of Chondrogenic Commitment of Human Adipose-Derived Stem CellsReportar como inadecuado




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1

3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Taipas, Guimarães 4806-909, Portugal

2

ICVS-3B’s—PT Government Associate Laboratory, Braga-Guimarães, Portugal





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Author to whom correspondence should be addressed.



Academic Editor: Esmaiel Jabbari

Abstract Magnetic nanoparticles MNPs are attractive tools to overcome limitations of current regenerative medicine strategies, demonstrating potential to integrate therapeutic and diagnostic functionalities in highly controlled systems. In traditional tissue engineering TE approaches, the MNPs association with stem cells in a three-dimensional 3D template offers the possibility to achieve a mechano-magnetic responsive system, enabling remote control actuation. Herein, we propose to study the role of MNPs integrated in κ-carrageenan κC hydrogels in the cellular response of human adipose-derived stem cells hASCs aiming at cartilage TE applications. The results indicated that the concentration of MNPs in the κC hydrogels influences cellular behavior, tuning a positive effect on cell viability, cell content and metabolic activity of hASCs, with the most promising outcomes found in 5% MNP-κC matrices. Although hASCs laden in MNPs-free- and MNPs-κC hydrogels showed similar metabolic and proliferation levels, MNPs κC hydrogels under magnetic actuation evidenced an instructive effect on hASCs, at a gene expression level, towards chondrogenic phenotype even in basic medium cultures. Therefore, the MNPs-based systems developed in this study may contribute to advanced strategies towards cartilage-like engineered substitutes. View Full-Text

Keywords: sulfated polysaccharides; magnetic nanoparticles; magnetic tissue engineering; stem cells; chondrogenic commitment; cartilage sulfated polysaccharides; magnetic nanoparticles; magnetic tissue engineering; stem cells; chondrogenic commitment; cartilage





Autor: Elena G. Popa 1,2, Vítor E. Santo 1,2, Márcia T. Rodrigues 1,2 and Manuela E. Gomes 1,2,*

Fuente: http://mdpi.com/



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