Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock ResuscitationReportar como inadecuado


Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock Resuscitation


Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock Resuscitation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China

2

Department of General Surgery, South Medical University, Guangzhou 510515, China





*

Author to whom correspondence should be addressed.



Abstract n-3 polyunsaturated fatty acids PUFAs can improve the function of the intestinal barrier after damage from ischemia-reperfusion or hemorrhagic shock resuscitation HSR. However, the effects of n-3 PUFAs on intestinal microbiota and the innate immunity of the intestinal mucosa after HSR remain unclear. In the present study, 40 C57BL-6J mice were randomly assigned to five groups: control, sham, HSR, HSR + n-3 PUFAs and HSR + n-6 PUFAs. Mice were sacrificed 12 h after HSR. Liver, spleen, mesenteric lymph nodes and terminal ileal tissues were collected. Intestinal mucosae were scraped aseptically. Compared with the HSR group, the number of goblet cells increased, expression of mucin 2 was restored and disturbed intestinal microbiota were partly stabilized in the PUFA-administered groups, indicating that both n-3 and n-6 PUFAs reduced overproliferation of Gammaproteobacteria while promoting the growth of Bacteroidetes. Notably, n-3 PUFAs had an advantage over n-6 PUFAs in improving ileal tissue levels of lysozyme after HSR. Thus, PUFAs, especially n-3 PUFAs, partly improved the innate immunity of intestinal mucosa in mice after HSR. These findings suggest a clinical rationale for providing n-3 PUFAs to patients recovering from ischemia-reperfusion. View Full-Text

Keywords: n-3 PUFAs; hemorrhagic shock; intestinal mucosa; innate immunity; intestinal microbiota; intestinal barrier function n-3 PUFAs; hemorrhagic shock; intestinal mucosa; innate immunity; intestinal microbiota; intestinal barrier function





Autor: Feng Tian 1,†, Xuejin Gao 1,†, Li Zhang 1, Xinying Wang 1,* , Xiao Wan 1, Tingting Jiang 1, Chao Wu 1, Jingcheng Bi 1 and Qiucheng Lei 1,2

Fuente: http://mdpi.com/



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