Facile Attachment of TAT Peptide on Gold Monolayer Protected Clusters: Synthesis and CharacterizationReportar como inadecuado


Facile Attachment of TAT Peptide on Gold Monolayer Protected Clusters: Synthesis and Characterization


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1

Advanced Materials Division, DST-Mintek Nanotechnology Innovation Centre, Mintek, Private Bag X3015, Randburg 2125, South Africa

2

Department of Chemistry, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, South Africa



Present Address: Sasol Pty Ltd., P.O. Box 1, Sasolburg 1947, South Africa



Present Address: CSIR Materials Science and Manufacturing, P.O. Box 395, Pretoria 0001, South Africa





*

Authors to whom correspondence should be addressed.



Academic Editor: Thomas Nann

Abstract High affinity thiolate-based polymeric capping ligands are known to impart stability onto nanosized gold nanoparticles. Due to the stable gold-sulfur bond, the ligand forms a protective layer around the gold core and subsequently controls the physicochemical properties of the resultant nanogold mononuclear protected clusters AuMPCs. The choice of ligands to use as surfactants for AuMPCs largely depends on the desired degree of hydrophilicity and biocompatibility of the MPCs, normally dictated by the intended application. Subsequent surface modification of AuMPCs allows further conjugation of additional biomolecules yielding bilayer or multilayered clusters suitable for bioanalytical applications ranging from targeted drug delivery to diagnostics. In this study, we discuss our recent laboratory findings on a simple route for the introduction of Trans-Activator of Transcription TAT peptide onto the surface of biotin-derivatised gold MPCs via the biotin-strepavidin interaction. By changing the surface loading of biotin, controlled amounts of TAT could be attached. This bioconjugate system is very attractive as a carrier in intercellular delivery of various delivery cargoes such as antibodies, proteins and oligonucleotides. View Full-Text

Keywords: nanoparticles; MPCs; gold; TAT; peptide; biotin nanoparticles; MPCs; gold; TAT; peptide; biotin





Autor: Ndabenhle M. Sosibo 1,2,†, Frankline K. Keter 1, Amanda Skepu 1, Robert T. Tshikhudo 1,‡,* and Neerish Revaprasadu 2,*

Fuente: http://mdpi.com/



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