An Investigation of the Cytotoxicity and Caspase-Mediated Apoptotic Effect of Green Synthesized Zinc Oxide Nanoparticles Using Eclipta prostrata on Human Liver Carcinoma CellsReportar como inadecuado


An Investigation of the Cytotoxicity and Caspase-Mediated Apoptotic Effect of Green Synthesized Zinc Oxide Nanoparticles Using Eclipta prostrata on Human Liver Carcinoma Cells


An Investigation of the Cytotoxicity and Caspase-Mediated Apoptotic Effect of Green Synthesized Zinc Oxide Nanoparticles Using Eclipta prostrata on Human Liver Carcinoma Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 05029, Korea

2

Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam 632509, Vellore District, Tamil Nadu, India





*

Author to whom correspondence should be addressed.



Academic Editor: Robert Tanguay

Abstract Cancer is a leading cause of death worldwide and sustained focus is on the discovery and development of newer and better tolerated anticancer drugs, especially from plants. In the present study, a simple, eco-friendly, and inexpensive approach was followed for the synthesis of zinc oxide nanoparticles ZnO NPs using the aqueous leaf extract of Eclipta prostrata. The synthesized ZnO NPs were characterized by UV-visible absorption spectroscopy, X-ray diffraction XRD, Fourier transform infrared spectroscopy FTIR, Scanning electron microscopy with energy dispersive X-ray spectroscopy SEM-EDX, High-resolution transmission electron microscopy HRTEM, and Selected area electron diffraction SAED. The HRTEM images confirmed the presence of triangle, radial, hexagonal, rod, and rectangle, shaped with an average size of 29 ± 1.3 nm. The functional groups for synthesized ZnO NPs were 3852 cm−1 for H-H weak peak, 3138 cm−1 for aromatic C-H extend, and 1648 cm−1 for Aromatic ring stretch. The 3-4,5-Dimethylthiazol-2-yl-2,5-Diphenyltetrazolium Bromide MTT, caspase and DNA fragmentation assays were carried out using various concentrations of ZnO NPs ranging from 1 to 100 mg-mL. The synthesized ZnO NPs showed dose dependent cytopathic effects in the Hep-G2 cell line. At 100 mg-mL concentration, the synthesized ZnO NPs exhibited significant cytotoxic effects and the apoptotic features were confirmed through caspase-3 activation and DNA fragmentation assays. View Full-Text

Keywords: anticancer actions; caspase study; zinc oxide nanoparticles; Eclipta prostrata anticancer actions; caspase study; zinc oxide nanoparticles; Eclipta prostrata





Autor: Ill-Min Chung 1, Abdul Abdul Rahuman 2, Sampath Marimuthu 2, Arivarasan Vishnu Kirthi 2, Karunanithi Anbarasan 2 and Govindasamy Rajakumar 1,*

Fuente: http://mdpi.com/



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