Utilization of Enzyme-Immobilized Mesoporous Silica Nanocontainers IBN-4 in Prodrug-Activated Cancer TheranosticsReportar como inadecuado


Utilization of Enzyme-Immobilized Mesoporous Silica Nanocontainers IBN-4 in Prodrug-Activated Cancer Theranostics


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1

Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien-974, Taiwan

2

Department of Chemistry, Kakatiya University, Telangana State-506009, India

3

Department of Chemistry, Tamkang University, New Taipei City 251, Taiwan



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editors: Jordi Sort and Eva Pellicer

Abstract To develop a carrier for use in enzyme prodrug therapy, Horseradish peroxidase HRP was immobilized onto mesoporous silica nanoparticles IBN-4: Institute of Bioengineering and Nanotechnology, where the nanoparticle surfaces were functionalized with 3-aminopropyltrimethoxysilane and further conjugated with glutaraldehyde. Consequently, the enzymes could be stabilized in nanochannels through the formation of covalent imine bonds. This strategy was used to protect HRP from immune exclusion, degradation and denaturation under biological conditions. Furthermore, immobilization of HRP in the nanochannels of IBN-4 nanomaterials exhibited good functional stability upon repetitive use and long-term storage 60 days at 4 °C. The generation of functionalized and HRP-immobilized nanomaterials was further verified using various characterization techniques. The possibility of using HRP-encapsulated IBN-4 materials in prodrug cancer therapy was also demonstrated by evaluating their ability to convert a prodrug indole-3- acetic acid IAA into cytotoxic radicals, which triggered tumor cell apoptosis in human colon carcinoma HT-29 cell line cells. A lactate dehydrogenase LDH assay revealed that cells could be exposed to the IBN-4 nanocomposites without damaging their membranes, confirming apoptotic cell death. In summary, we demonstrated the potential of utilizing large porous mesoporous silica nanomaterials IBN-4 as enzyme carriers for prodrug therapy. View Full-Text

Keywords: mesoporous silica nanoparticles; enzyme immobilization; Institute of Bioengineering and Nanotechnology IBN-4; anti-cancer; prodrug therapy mesoporous silica nanoparticles; enzyme immobilization; Institute of Bioengineering and Nanotechnology IBN-4; anti-cancer; prodrug therapy





Autor: Bau-Yen Hung 1,†, Yaswanth Kuthati 1,†, Ranjith Kumar Kankala 1,†, Shravankumar Kankala 2, Jin-Pei Deng 3, Chen-Lun Liu 1 and Chia-Hung Lee 1,*

Fuente: http://mdpi.com/



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