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1

Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab. for Biomass Chemical Utilization; Key and Open Lab. on Forest Chemical Engineering, SFA; Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

2

Institute of New Technology of Forestry, CAF, Beijing 100091, China





*

Author to whom correspondence should be addressed.



Abstract Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl DPPH free radical-scavenging and ferric-reducing antioxidant power FRAP assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 °C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

Keywords: Acacia mangium; phenolic compounds; condensed tannins; antioxidant activities; central composite design Acacia mangium; phenolic compounds; condensed tannins; antioxidant activities; central composite design





Autor: Liangliang Zhang 1,2,* , Jiahong Chen 1, Yongmei Wang 1,2, Dongmei Wu 1,2 and Man Xu 1

Fuente: http://mdpi.com/



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