Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed ExtractReportar como inadecuado




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1

Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia

2

Institute for Research in Molecular Medicine INFORMM, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia

3

Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia

4

Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia





*

Author to whom correspondence should be addressed.



Abstract Background and objective: Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation. Results: The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography TLC and liquid chromatography-mass spectrometry LC-MS. The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone. Conclusions: The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.

Keywords: bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation





Autor: Subramanion L. Jothy 1, Zuraini Zakaria 1, Yeng Chen 2,3,* , Yee Ling Lau 4, Lachimanan Yoga Latha 2, Lai Ngit Shin 2 and Sreenivasan Sasidharan 2

Fuente: http://mdpi.com/



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