Camptothecin-20s-O-N-3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan-lysine, a Novel Camptothecin Analogue, Induces Apoptosis towards Hepatocellular Carcinoma SMMC-7721 CellsReportar como inadecuado




Camptothecin-20s-O-N-3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan-lysine, a Novel Camptothecin Analogue, Induces Apoptosis towards Hepatocellular Carcinoma SMMC-7721 Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin 150040, China





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Abstract Camptothecin-20s-O-N-3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan-lysine B2 is a novel camptothecin analogue. Our previous study had shown that it displayed higher cytoxicity activity towards hepatocellular carcinoma SMMC-7721 cells than camptothecin CPT in vitro. In this paper, the underlying mechanism of anti-proliferation of B2 towards SMMC-7721 cells was further examined. Cell growth inhibition of B2 was determined using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay; morphological changes were observed under Laser Scanning Confocal Microscope LSCM; cell cycle distribution, apoptotic population, changes in mitochondrial membrane potential, intracellular calcium concentration and reactive oxygen species ROS production were determined by flow cytometry FCM. Activities of caspase-3 and caspase-9 were measured, and the expression level of Bcl-2 and Bax proteins were analyzed by Western blot. The results suggested that B2 inhibited SMMC-7721 cell growth by causing cell cycle arrest at the S and G2-M phases, and induced apoptosis involving a mitochondrial pathway. B2 appears to cause a high induction of apoptosis on SMMC-7721 cells in vitro, which suggests it might be a potential drug for cancer therapy.

Keywords: camptothecin analogue; apoptosis; hepatocellular carcinoma SMMC-7721 cell camptothecin analogue; apoptosis; hepatocellular carcinoma SMMC-7721 cell





Autor: Qingyong Li * , Wei Qiu, Qiaochu Zhu, Yuangang Zu, Xiaoqiu Deng, Tengfei Zhao, Chunfei Jiang and Li Zhang

Fuente: http://mdpi.com/



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