Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current ChromatographyReportar como inadecuado




Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

College of Pharmacy, Harbin University, Daqing 163319, China

2

Petrochemical Research Institute Daqing Petrochemical Research Center, Daqing 163714, China





*

Author to whom correspondence should be addressed.



Abstract Semi-preparative high-speed counter-current chromatography HSCCC was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water 2.8:5:2.8:5, v-v-v-v. The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography HPLC. A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, 1H-NMR and 13C-NMR.

Keywords: Isodon rubescens; high-speed counter-current chromatography HSCCC; oridonin Isodon rubescens; high-speed counter-current chromatography HSCCC; oridonin





Autor: Fa He 1, Yuhua Bai 1,* , Jing Wang 1, Jing Wei 2, ChunYue Yu 1, Sen Li 1, Weili Yang 1 and Chenghua Han 1

Fuente: http://mdpi.com/



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