Structural Characterization and Identification of Major Constituents in Jitai Tablets by High-Performance Liquid Chromatography-Diode-Array Detection Coupled with Electrospray Ionization Tandem Mass SpectrometryReportar como inadecuado




Structural Characterization and Identification of Major Constituents in Jitai Tablets by High-Performance Liquid Chromatography-Diode-Array Detection Coupled with Electrospray Ionization Tandem Mass Spectrometry - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2

College of Pharmacy, Nankai University, Tianjin 300071, China

3

National Engineering Research Center for TCM, Shanghai 201203, China

4

School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Abstract In the present study a universally applicable HPLC-DAD-ESI-MS-MS method was developed for carrying out the comprehensive characterization of Jitai tablets JTT. Based on the ESI-MSn fragmentation patterns of the reference standards, a total of 101 components were identified or tentatively characterized by comparing their retention times, UV and MS spectra with those of reference standards or through the matching of empirical information with those of published components in the in-house library. The characteristic fragmentation pattern of alkaloids, phenolic acids, tanshinones, flavonoid glycosides, cyanogenic glycosides, ginsenosides, 2-2-phenylethyl chromones, phthalides and gingerol-related compounds were tentatively elucidated using structurally-relevant product ions. It was observed that neutral losses of C9H10O3 and C9H8O2 were the characteristic product ions of scopola alkaloids. Neutral fragment mandelonitrile was the characteristic ion of cyanogenic glycosides. To our knowledge, tropylium ion and C4H2O unit were the characteristic ions of 2-2-phenylethyl chromone, which resulted from the Retro-Diels-Alder RDA cleavage of the C ring. The results indicated that the developed analysis method could be employed as a rapid, effective technique for structural characterization of chemical constituents in TCM. This work is expected to provide comprehensive information for the quality evaluation and pharmacokinetic studies of JTT.

Keywords: constituents identification; TCM; HPLC-DAD-ESI-MS-MS; Jitai tablet constituents identification; TCM; HPLC-DAD-ESI-MS-MS; Jitai tablet





Autor: Shuping Wang 1,†, Lei Liu 2,†, Lingling Wang 3, Yaohua Hu 1, Weidong Zhang 1,4,* and Runhui Liu 1,*

Fuente: http://mdpi.com/



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