Specific Binding of Anionic Porphyrin and Phthalocyanine to the G-Quadruplex with a Variety of in Vitro and in Vivo ApplicationsReportar como inadecuado




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1

Advanced Technology Research Laboratories, Panasonic Corporation, 3-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan

2

Frontier Institute for Biomolecular Engineering Research FIBER, Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan

3

Faculty of Frontiers of Innovative Research in Science and Technology FIRST, Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan





*

Author to whom correspondence should be addressed.



Abstract The G-quadruplex, a four-stranded DNA structure with stacked guanine tetrads G-quartets, has recently been attracting attention because of its critical roles in vitro and in vivo. In particular, the G-quadruplex functions as ligands for metal ions and aptamers for various molecules. Interestingly, the G-quadruplex can show peroxidase-like activity with an anionic porphyrin, iron III protoporphyrin IX hemin. Importantly, hemin binds to G-quadruplexes with high selectivity over single-stranded DNA ssDNA and double-stranded DNA dsDNA, which is attributable to an electrostatic repulsion of phosphate groups in ssDNA and dsDNA. The G-quadruplex and hemin-G-quadruplex complex allow development of sensing techniques to detect DNA, metal ions and proteins. In addition to hemin, anionic phthalocyanines also bind to the G-quadruplex formed by human telomere DNA, specifically over ssDNA and dsDNA. Since the binding of anionic phthalocyanines to the G-quadruplex causes an inhibition of telomerase activity, which plays a role in the immortal growth of cancer cells, anionic phthalocyanines are promising as novel anticancer drug candidates. This review focuses on the specific binding of hemin and anionic phthalocyanines to G-quadruplexes and the applications in vitro and in vivo of this binding property.

Keywords: anionic phthalocyanine; DNAzyme; G-quadruplex; hemin; peroxidase; telomerase anionic phthalocyanine; DNAzyme; G-quadruplex; hemin; peroxidase; telomerase





Autor: Hidenobu Yaku 1,2,3,* , Takashi Murashima 2,3, Daisuke Miyoshi 2,3 and Naoki Sugimoto 2,3

Fuente: http://mdpi.com/



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