Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosaReportar como inadecuado




Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China

2

Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

3

College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China





*

Author to whom correspondence should be addressed.



Abstract Twenty-five novel EST-derived simple sequence repeat EST-SSR markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci. The numbers of alleles per locus ranged from 4 to 14, with an average of 8.08. The observed and expected heterozygosities varied from 0.2917 to 1.000 and from 0.3570 to 0.9002, respectively. After sequential Bonferroni correction for multiple tests, only one locus was found to deviate from Hardy-Weinberg equilibrium. Twenty-five EST-SSR markers showed a high rate of across-species transferability 100% in Scapharca subcrenata and a low rate of across-genus transferability 20% in Tegillarca granosa. These EST-SSRs will be helpful for QTL mapping, molecular breeding and investigation of population genetic diversity in ark shell S. broughtonii and other Scapharca species.

Keywords: ark shell; Scapharca broughtonii; EST-SSR markers; transferability ark shell; Scapharca broughtonii; EST-SSR markers; transferability





Autor: Meng Li 1,2, Ling Zhu 2,* , Chun-Ya Zhou 2,3, Lin Lin 2, Yan-Jun Fan 2 and Zhi-Meng Zhuang 2

Fuente: http://mdpi.com/



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