Protective Effects of the Key Compounds Isolated from Corni fructus against β-Amyloid-Induced Neurotoxicity in PC12 CellsReportar como inadecuado




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1

Department of Food Science and Nutrition, Dong-A University, Busan 604-714, Korea

2

Department of Food & Life Sciences, Inje University, Gimhae, Gyeongnam 621-749, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract β-Amyloid Aβ peptide is the major component of senile plaques and is considered to have a causal role in the development and progression of Alzheimer’s disease AD. There is compelling evidence supporting the notion that Aβ-induced cytotoxicity is mediated though the generation of ROS. In the present study, we investigated the neuroprotective effects of ursolic acid UA, p-coumaric acid p-CA, and gallic acid GA isolated from Corni fructus CF against Aβ25–35-induced toxicity in PC12 cell. Exposure of PC12 cells to 50 μM Aβ25–35 increased cellular oxidative stress, the number of apoptotic cells and caspase-3 activity and finally caused significant cell death. However, UA, p-CA, and GA not only suppressed the generation of ROS but also attenuated DNA fragmentation and eventually attenuated Aβ-induced apoptosis in a dose-dependent manner. In protecting cells against Aβ neurotoxicity, UA and GA possessed stronger ability against ROS generation than p-CA, while p-CA showed the strongest anti-apoptotic activity. Particularly, p-CA protected cells at the concentration range from 0.5 up to 125 μM without any adverse effect. Taken together, these effects of UA, p-CA, and GA may be partly associated with the neuroprotective effect of CF. Furthermore, our findings might raise a possibility of therapeutic applications of CF for preventing and-or treating neurodegenerative diseases.

Keywords: Alzheimer’s disease AD; amyloid β peptide Aβ; Corni fructus; apoptosis; anti-oxidant; neuroprotection Alzheimer’s disease AD; amyloid β peptide Aβ; Corni fructus; apoptosis; anti-oxidant; neuroprotection





Autor: Seung-Young Hong 1,†, Woo-Sik Jeong 2,† and Mira Jun 1,*

Fuente: http://mdpi.com/



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