Lipid Classes and Fatty Acid Regiodistribution in Triacylglycerols of Seed Oils of Two Sambucus Species S. nigra L. and S. ebulus L.Reportar como inadecuado




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1

Department of Environmental and Plant Protection, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca 400372, Manastur 3-5, Romania

2

Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca 400372, Manastur 3-5, Romania

3

Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca 400372, Manastur 3-5, Romania





*

Author to whom correspondence should be addressed.



Abstract The oil content and fatty acid composition of total lipids TLs and main lipid classes NLs- neutral and PLs- polar lipids in seeds of two wild Sambucus species S. nigra and S. ebulus from Transylvania Romania were determined by capillary gas chromatography GC-MS. In addition, the positional distribution of fatty acids in seed triacylglycerols TAGs was determined by hydrolysis with pancreatic lipase. The seeds were found to be rich in fat 22.40–24.90 g-100g with high amounts of polyunsaturated fatty acids PUFAs ranging from 68.96% S. ebulus to 75.15% S. nigra. High ratios of PUFAs-SFAs saturated fatty acids, ranging from 7.06 S. nigra to 7.64 S. ebulus, and low ratios of n-6-n-3, ranging from 0.84 S. nigra to 1.51 S. ebulus, were determined in both oils. The lipid classes-subclasses analyzed PLs, MAGs—monoacylglycerols, DAGs—diacylglycerols, FFAs—free fatty acids, TAGs and SEs—sterol esters were separated and identified using thin-layer chromatography. The fatty acid compositions of the TAG fractions were practically identical to the profiles of TLs, with the same dominating fatty acids in both analyzed species. SEs and FFAs, were characterized by high proportions of SFAs. The sn-2 position of TAGs was esterified predominantly with linoleic acid 43.56% for S. nigra and 50.41% for S. ebulus. View Full-Text

Keywords: Sambucus seed oils; fatty acid composition; lipid class; stereospecific analysis; GC-MS Sambucus seed oils; fatty acid composition; lipid class; stereospecific analysis; GC-MS





Autor: Francisc Vasile Dulf 1, Ioan Oroian 1, Dan Cristian Vodnar 2,* , Carmen Socaciu 2 and Adela Pintea 3

Fuente: http://mdpi.com/



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