Active Components with Inhibitory Activities on IFN-γ-STAT1 and IL-6-STAT3 Signaling Pathways from Caulis TrachelospermiReportar como inadecuado




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1

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China

2

Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China





*

Author to whom correspondence should be addressed.



Abstract Initial investigation for new active herbal extract with inhibiting activity on JAK-STAT signaling pathway revealed that the extract of Caulis Trachelospermi, which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography, was founded to strongly inhibit IFN-γ-induced STAT1-responsive luciferase activity IFN-γ-STAT1 with IC50 value of 2.43 μg-mL as well as inhibiting IL-6-induced STAT3-responsive luciferase activity IL-6-STAT3 with IC50 value of 1.38 μg-mL. Subsequent study on its active components led to the isolation and identification of two new dibenzylbutyrolactone lignans named 4-demethyltraxillaside 1 and nortrachelogenin 4-O-β-d-glucopyranoside 2, together with six known compounds. The lignan compounds 1–4 together with other lignan compounds isolated in previous study were tested the activities on IFN-γ-STAT1 and IL-6-STAT3 pathways. The following result showed that the main components trachelogenin and arctigenin had corresponding activities on IFN-γ-STAT1 pathway with IC50 values of 3.14 μM and 9.46 μM as well as trachelogenin, arctigenin and matairesinol strongly inhibiting IL-6-STAT3 pathway with IC50 values of 3.63 μM, 6.47 μM and 2.92 μM, respectively. View Full-Text

Keywords: Caulis Trachelospermi; 4-demethyltraxillaside; nortrachelogenin 4-O-β-d-glucopyranoside; JAK-STAT signaling pathway Caulis Trachelospermi; 4-demethyltraxillaside; nortrachelogenin 4-O-β-d-glucopyranoside; JAK-STAT signaling pathway





Autor: Xiao-Ting Liu 1,2, Zhe-Xing Wang 1, Yu Yang 2, Lin Wang 2, Ruo-Feng Sun 2, Yi-Min Zhao 2 and Neng-Jiang Yu 2,*

Fuente: http://mdpi.com/



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