Ultrasound-Assisted Extraction of Antioxidants in Misai Kucing Orthosiphon stamineusReportar como inadecuado




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1

Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia

2

School of Science, Monash University Malaysia, Bandar Sunway, Petaling Jaya 46150, Selangor, Malaysia

3

Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia

4

Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras 56000, Kuala Lumpur, Malaysia





*

Author to whom correspondence should be addressed.



Abstract Ultrasound-assisted extraction UAE with ethanol was used to extract the compounds responsible for the antioxidant activities of Misai Kucing Orthosiphon stamineus. Response surface methodology RSM was used to optimize four independent variables: ethanol concentration %, amplitude %, duty cycle W-s and extraction time min. Antioxidant compounds were determined by total phenolic content and total flavonoid content to be 1.4 g gallic acid equivalent-100 g DW and 45 g catechin equivalent-100 g DW, respectively. Antioxidant activities were evaluated using the 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid ABTS•+ radical scavenging capacity assay and the 2,2-diphenyl-1-picrylhydrazyl DPPH• radical scavenging capacity assay to be 1,961.3 and 2,423.3 µmol Trolox Equivalent Antioxidant Capacity TEAC-100 g DW, respectively. Based on the optimal conditions, experimental values were reported to be close to the predicted value by RSM modeling p > 0.05, indicating the suitability of UAE for extracting the antioxidants of Misai Kucing. Rosmarinic acid, kaempferol-rutinoside and sinesetine were identified by high performance liquid chromatography-mass spectrometry. View Full-Text

Keywords: ultrasound; extraction; antioxidants; Misai Kucing; Orthosiphon stamineus ultrasound; extraction; antioxidants; Misai Kucing; Orthosiphon stamineus





Autor: Swee Kheng Ho 1, Chin Ping Tan 1,* , Yin Yin Thoo 2, Faridah Abas 3 and Chun Wai Ho 4

Fuente: http://mdpi.com/



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