Development and Characterization of Polymorphic Genic-SSR Markers in Larix kaempferiReportar como inadecuado




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1

State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, China

2

Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, China





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Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract New simple sequence repeat SSR markers were developed in the Japanese larch Larix kaempferi using unigene sequences for further genetic diversity studies and the genetic improvement of breeding programs. One thousand two handred and thirty five 1235 primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the amplified candidate markers, 145 90.6% exhibited polymorphism among 24 individuals of L. kaempferi, with the number of alleles per locus Na, observed heterozygosity Ho, expected heterozygosity He and polymorphic information content PIC averaging at 4.510, 0.487, 0.518 and 0.459, respectively. Cross-species amplification of randomly selection of 30 genic-SSRs among the 145 polymorphic ones showed that 80.0% of the SSRs could be amplified in Larix olgensis, 86.7% could be amplified in Larix principi-rupprechtii and 83.0% could be amplified in Larix gmelinii. High rates of cross-species amplification were observed. The genic-SSRs developed herein would be a valuable resource for genetic analysis of Larix kaempferi and related species, and also have the potential to facilitate the genetic improvement and breeding of larch. View Full-Text

Keywords: Larix kaempferi; unigene sequences; simple sequence repeat SSR; polymorphism; cross-species amplification Larix kaempferi; unigene sequences; simple sequence repeat SSR; polymorphism; cross-species amplification





Autor: Xing-Bin Chen 1,2, Yun-Hui Xie 1,2 and Xiao-Mei Sun 1,2,*

Fuente: http://mdpi.com/



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